COMPARATIVE EFFECTS OF EGG YOLKS FROM DIFFERENT POULTRY BREEDS ON VIABILITY OF REFRIGERATED SPERMATOZOA FROM WEST AFRICAN DWARF BUCKS
DOI:
https://doi.org/10.51406/jagse.v13i1.1207Keywords:
Buck, egg yolks, refrigerated spermatozoa, viabilityAbstract
Egg yolk from avian species is used as a common component of most semen extenders because of its wide availability, beneficial effect on sperm viability and protective effect on acrosome against temperature-related damage during semen preservation. This study was carried out to determine the comparative effects of egg yolks from different poultry breeds on viability of refrigerated spermatozoa of West African Dwarf (WAD) bucks. Pooled semen from five intact WAD bucks was diluted with extender containing egg yolks from Normal feather (NF), Nickel neck (NN), Frizzle feather (FF), Nera black (NB), Oba Marshall black (OB) and Yaffa brown (YB) chickens. Following dilution, the semen samples were assessed subjectively after in vitro storage at 5oC for 24, 48, 72 and 96 hours as regards progressive sperm motility, acrosome status and abnormalities using a phase-contrast microscope. The results showed that the ability of these egg yolks to sustain progressive motility ranked in this order: OB > NN > NF >YB > NB > FF. Intact acrosome was comparable during the 24, 48, 72 and 96 hours of storage and followed similar trend across the egg-yolk types in the extender. Extender containing YB had the highest percentage abnormality at the end of this study. The findings of this study reveal that OB, NN and NF have better protective ability to maintain motility of refrigerated spermatozoa of WAD bucks.
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References
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Bathgate R., Maxwell W.M.C., Evans G. 2006 – Studies on the effects of supplementing boar semen cryopreservation media with different avian egg yolk types on in vitro post-thaw sperm quality. Reproduction in Domestic Animals, 41, 68-73.
Bearden, H.J., Fuquay, J.W. 1997. Semen evaluation. In: Applied animal reproduction. 4th ed. New Jersey: Prentice Hall; pp. 158-169.
Bergeron, A., Manjunath, P. 2006 – New insights towards understanding the mechanisms of sperm protection by egg yolk and milk. Molecular Reproduction and Development, 73, 1338-1344.
Bitto, I. I., Egbunike, G. N. 2006. Seasonal variations in sperm production, gonadal and extragonadal sperm reserves in pubertal West African dwarf bucks in their native tropical environment. Livestock Research for Rural Development, 18 (9), www.lrrd.org/lrrd18/9/bitt18134.htm
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Choi, S.H., Song, K.T., Oh, H.R. 2001. Cholesterol contents and fatty acid composition of chukar, pheasant, guinea fowl and quail egg yolk. Asian-Australasian Journal of Animal Sciences, 14:831–836.
Clulow, J.R., Maxwell, W.M.C., Evans, G., Morris, L.H.A. 2007. A comparision of duck and chicken egg yolk fort he cryopreservation of stallion sperm. Australian Veterinary Journal, 85:232–235.
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Iritani, A., Nishikawa, Y. 1961. Studies on the eggâ€yolk coagulating factors in goat semen: II Properties of the coagulating factor and influential conditions for coagulation. In: Proc. Silver Jubilee Lab. Anim. Husbandry, Kyoto University, pp. 97–104.
Kulaksiz, R., Cebi, C., Akcay, E., Daskin, A. 2010. The protective effect of egg yolk from different avian species during the cryopreservation of Karayaka ram semen. Small Ruminant Research, 88: 12-15.
Manjunath P., Nauc V., Bergeron A., Menard M. 2002 – Major proteins of bovine seminal plasma bind to the low-density lipoproteins fraction of hen’s eggs yolk. Biology of Reproduction, 67:1250-1258.
Maurice, D.V., Lightsey, S.F., Hsu, K.T., Gaylord, T.G., Reedy, R.V. 1994. Cholesterol in eggs from different species of poultry determined by capillary GLC. Food Chemistry, 50:367–372.
Pace, M.M., Graham, E.F. 1974. The components in egg yolk which protect bovine spermatozoa during freezing. Journal of Animal Science, 39: 1144–1149.
Prasard, R.V., Sreenivas Reedy, M., Dhananjaya Reedy, B., Rao, P.V.A. 1988. A comparative study on the quality of fresh chicken and duck eggs. Indian Journal of Animal Science, 8: 978–981.
Recai, K., Cigdem, C.，Ergun, A.，Ali, .D. 2010.The protective effect of egg yolk from different avian species during the cryopreservation of Karayaka ram semen. Small Ruminant Research, 88:12–15
Rocha, A., Oliveira E., Diaz J.K., Sousa M. 2006 – A novel apical midpiece defect in the spermatozoa of a bull without an apparent decrease in motility and fertility: a case study. Theriogenology, 66: 913-922.
Su, L., Li X., Quan J., Yang S., Li Y., He X., Tang X. 2008 – A comparison of the protective action of added egg yolks from five avian species to the cryopreservation of bull sperm. Animal Reproduction Science, 104: 212-219.
Surai, P.F., Speake, B.K., Noble, R.C., Mezes, M. 1999. Species-specific differences in the fatty acid profiles of the lipids of the yolk and of the liver of the chick. Journal of the Science of Food and Agriculture., 79: 733-736.
Thomas C.A., Garner D.L., Jarnette J.M., Marshall C.E. 1997 – Fluorometric assessment of acrosomal integrity and viability in cryopreserved bovine spermatozoa. Biology of Reproduction, 45:880-887.
Trimeche A., Anton M., Renard P., Gandemer G., Tainturier D. 1997 – Quail egg yolk: A novel cryoprotectants for the freeze preservation of Poitou jackass sperm. Cryobiology, 34: 385-393.
Trimeche A., Renard P., Tainturier D. 1998 – A procedure for Poitou jackass sperm cryopreservation, Theriogenology, 50: 793-806.
Wassarman, P.M. 1988. Zonal pellucid glycoproteins. Journal Biological Chemistry. 283: 24285-24289.
Watson, P.F. 1976. The protection of ram and bull spermatozoa by the low density lipoprotein fraction of egg yolk during storage at 5 ◦C and deep freezing. Journal of Thermal Biology. 1: 137–141.
Amirat, L., Tainturier, D., Jeanneau, L., Thorin, C., Gerard, O., Courtens, J.L., Anton, M. 2004. Bull semen in vitro fertility after cryopreservation using egg yolk LDL: a comparison with optidyl, a commercial egg yolk extender. Theriogenology, 61: 895–907.
Andrabi S.M.H., Ansari M.S., Ullah N., Anwar M., Mehmood A., Akhter S. 2008 – Duck egg yolk in extender improves the freezability of buffalo bull spermatozoa. Animal Reproduction Science, 104, 427-433.
Ansari, M.S., Rakha, B.A., Andrabi, S.M.H., Ullah, N., Akhter, S. 2010. Cryopreservation of Sahiwal bull epididymal spermatozoa. Pakistan Journal of Zoology, 42, 741-743.
Bair, C.W., Marion, W.W. 1978. Yolk cholesterol in eggs from various avian species. Poultry Science, 57: 1260-1265.
Bathgate R., Maxwell W.M.C., Evans G. 2006 – Studies on the effects of supplementing boar semen cryopreservation media with different avian egg yolk types on in vitro post-thaw sperm quality. Reproduction in Domestic Animals, 41, 68-73.
Bearden, H.J., Fuquay, J.W. 1997. Semen evaluation. In: Applied animal reproduction. 4th ed. New Jersey: Prentice Hall; pp. 158-169.
Bergeron, A., Manjunath, P. 2006 – New insights towards understanding the mechanisms of sperm protection by egg yolk and milk. Molecular Reproduction and Development, 73, 1338-1344.
Bitto, I. I., Egbunike, G. N. 2006. Seasonal variations in sperm production, gonadal and extragonadal sperm reserves in pubertal West African dwarf bucks in their native tropical environment. Livestock Research for Rural Development, 18 (9), www.lrrd.org/lrrd18/9/bitt18134.htm
Burris, C., Webb, G. 2009. Effects of egg yolk source on the cryopreservation of stallion semen. Journal Equine Veterinary Science, 29:336–337.
Choi, S.H., Song, K.T., Oh, H.R. 2001. Cholesterol contents and fatty acid composition of chukar, pheasant, guinea fowl and quail egg yolk. Asian-Australasian Journal of Animal Sciences, 14:831–836.
Clulow, J.R., Maxwell, W.M.C., Evans, G., Morris, L.H.A. 2007. A comparision of duck and chicken egg yolk fort he cryopreservation of stallion sperm. Australian Veterinary Journal, 85:232–235.
Duncan, D.B. 1955. Multiple Ranges and Multiples F-tests. Biometrics, 11:1–42.
Food and Agriculture Organization (FAO). 2006. A system of integrated agricultural censuses and surveys, volume 1, World Programme for the Census of Agriculture 2010. Statistical Development Series No 11.
Foulkes, J.A. 1977. The separation of lipoproteins from egg yolk and their effect on the motility and the integrity of bovine spermatozoa. Journal of Reproduction and Fertility, 49:277–284.
Henry, M., P. Neves. 1998. Manual para exame andrológico e avaliação de sêmen animal. 2th ed. Colégio Brasileiro de Reprodução Animal (CBRA), Belo Horizonte, MG
Humes, R., Webb, G. 2006. Use of chicken or chukar egg yolk with two cryoprotectants for preservation of stallion semen. Animal Reproduction Science, 94, 62–63.
Iritani, A., Nishikawa, Y. 1961. Studies on the eggâ€yolk coagulating factors in goat semen: II Properties of the coagulating factor and influential conditions for coagulation. In: Proc. Silver Jubilee Lab. Anim. Husbandry, Kyoto University, pp. 97–104.
Kulaksiz, R., Cebi, C., Akcay, E., Daskin, A. 2010. The protective effect of egg yolk from different avian species during the cryopreservation of Karayaka ram semen. Small Ruminant Research, 88: 12-15.
Manjunath P., Nauc V., Bergeron A., Menard M. 2002 – Major proteins of bovine seminal plasma bind to the low-density lipoproteins fraction of hen’s eggs yolk. Biology of Reproduction, 67:1250-1258.
Maurice, D.V., Lightsey, S.F., Hsu, K.T., Gaylord, T.G., Reedy, R.V. 1994. Cholesterol in eggs from different species of poultry determined by capillary GLC. Food Chemistry, 50:367–372.
Pace, M.M., Graham, E.F. 1974. The components in egg yolk which protect bovine spermatozoa during freezing. Journal of Animal Science, 39: 1144–1149.
Prasard, R.V., Sreenivas Reedy, M., Dhananjaya Reedy, B., Rao, P.V.A. 1988. A comparative study on the quality of fresh chicken and duck eggs. Indian Journal of Animal Science, 8: 978–981.
Recai, K., Cigdem, C.，Ergun, A.，Ali, .D. 2010.The protective effect of egg yolk from different avian species during the cryopreservation of Karayaka ram semen. Small Ruminant Research, 88:12–15
Rocha, A., Oliveira E., Diaz J.K., Sousa M. 2006 – A novel apical midpiece defect in the spermatozoa of a bull without an apparent decrease in motility and fertility: a case study. Theriogenology, 66: 913-922.
Su, L., Li X., Quan J., Yang S., Li Y., He X., Tang X. 2008 – A comparison of the protective action of added egg yolks from five avian species to the cryopreservation of bull sperm. Animal Reproduction Science, 104: 212-219.
Surai, P.F., Speake, B.K., Noble, R.C., Mezes, M. 1999. Species-specific differences in the fatty acid profiles of the lipids of the yolk and of the liver of the chick. Journal of the Science of Food and Agriculture., 79: 733-736.
Thomas C.A., Garner D.L., Jarnette J.M., Marshall C.E. 1997 – Fluorometric assessment of acrosomal integrity and viability in cryopreserved bovine spermatozoa. Biology of Reproduction, 45:880-887.
Trimeche A., Anton M., Renard P., Gandemer G., Tainturier D. 1997 – Quail egg yolk: A novel cryoprotectants for the freeze preservation of Poitou jackass sperm. Cryobiology, 34: 385-393.
Trimeche A., Renard P., Tainturier D. 1998 – A procedure for Poitou jackass sperm cryopreservation, Theriogenology, 50: 793-806.
Wassarman, P.M. 1988. Zonal pellucid glycoproteins. Journal Biological Chemistry. 283: 24285-24289.
Watson, P.F. 1976. The protection of ram and bull spermatozoa by the low density lipoprotein fraction of egg yolk during storage at 5 ◦C and deep freezing. Journal of Thermal Biology. 1: 137–141.
