PREVALENCE OF ANTIBIOTIC RESISTANCE UROPATHOGENIC Escherichia coli PRODUCING EXTENDED SPECTRUM BETA-LACTAMASE IN UNIVERSITY COLLEGE HOSPITAL, IBADAN, NIGERIA

Authors

  • W.E. IKE Department of Microbiology, College of Biosciences, Federal University of Agriculture Abeokuta, Abeokuta-Nigeria
  • T.O. ARIOM Department of Microbiology, College of Biosciences, Federal University of Agriculture Abeokuta, Abeokuta-Nigeria
  • A.O. AKINBOBOYE Department of Microbiology, College of Biosciences, Federal University of Agriculture Abeokuta, Abeokuta-Nigeria
  • A.O. ATAYESE Centre of Excellence in Agricultural Development and Sustainable Environment, Federal University of Agriculture, Abeokuta-Nigeria
  • B.A. MICHAEL Department of Microbiology, College of Biosciences, Federal University of Agriculture Abeokuta, Abeokuta-Nigeria
  • C.O. IGBOKWE Department of Pharmaceutical Microbiology and Biotechnology, Faculty of Pharmaceutical Sciences, University of Ibadan, Ibadan-Nigeria
  • O.B. SHITTU  Department of Microbiology, College of Biosciences, Federal University of Agriculture Abeokuta, Abeokuta-Nigeria

Keywords:

Antibiotic surveillance; Enzyme production; Urinary tract infection; Escherichia coli

Abstract

The global prevalence and general spread of Uropathogens producing extended spectrum beta-lactamase (ESBL) has remained a critical health concern globally. The rise in the prevalence of antimicrobial-resistant uropathogenic bacteria globally has also become a major health concern, due to the increasing mortality rate. However, one of the pathogens which has been classified as a priority pathogen by World Health Organisation is ESBL-producing Escherichia coli. This study was carried out to assess antibiotic resistance and ESBL production among E. coli recovered from the archive of University College Hospital, Ibadan, Nigeria. From a total of 330 isolates retrieved from the archive of the hospital, 129 isolates were presumptively identified as E. coli by standard phenotypic methods and confirmed by the use of Oxoid 12E MicrobactTM 2009 Gram-negative identification system. Majority of these isolates (67.4%) were Congo red positive and considered pathogenic. All the isolates were resistant to ampicillin, and tetracycline while significant percentage (95.5%) was resistant against gentamicin. 17.1% of the isolates demonstrated multidrug resistant phenotype with multiple antibiotic resistance indices range from 0.46 to 0.62. A mid-level rate (48%) of ESBL production was observed following double-disc synergy test. This study revealed high level of antibiotic resistant to ampicillin, tetracycline, and gentamicin with mid-level ESBL production, hence calling for routine antibiotic surveillance.

 

Author Biographies

W.E. IKE, Department of Microbiology, College of Biosciences, Federal University of Agriculture Abeokuta, Abeokuta-Nigeria

Department of Microbiology, College of Biosciences, Federal University of Agriculture Abeokuta, Abeokuta-Nigeria

 

T.O. ARIOM, Department of Microbiology, College of Biosciences, Federal University of Agriculture Abeokuta, Abeokuta-Nigeria

1Department of Microbiology, College of Biosciences, Federal University of Agriculture Abeokuta, Abeokuta-Nigeria

2Institute of Food Security, Environmental Resources, Federal University of Agriculture, Abeokuta, Abeokuta-Nigeria

 

 

A.O. AKINBOBOYE, Department of Microbiology, College of Biosciences, Federal University of Agriculture Abeokuta, Abeokuta-Nigeria

Department of Microbiology, College of Biosciences, Federal University of Agriculture Abeokuta, Abeokuta-Nigeria

 

A.O. ATAYESE, Centre of Excellence in Agricultural Development and Sustainable Environment, Federal University of Agriculture, Abeokuta-Nigeria

Department of Microbiology, College of Biosciences, Federal University of Agriculture Abeokuta, Abeokuta-Nigeria

Centre of Excellence in Agricultural Development and Sustainable Environment, Federal University of Agriculture, Abeokuta-Nigeria

 

B.A. MICHAEL, Department of Microbiology, College of Biosciences, Federal University of Agriculture Abeokuta, Abeokuta-Nigeria

Department of Microbiology, College of Biosciences, Federal University of Agriculture Abeokuta, Abeokuta-Nigeria

 

C.O. IGBOKWE, Department of Pharmaceutical Microbiology and Biotechnology, Faculty of Pharmaceutical Sciences, University of Ibadan, Ibadan-Nigeria

Department of Pharmaceutical Microbiology and Biotechnology, Faculty of Pharmaceutical Sciences, University of Ibadan, Ibadan-Nigeria

 

 

 

O.B. SHITTU , Department of Microbiology, College of Biosciences, Federal University of Agriculture Abeokuta, Abeokuta-Nigeria

Department of Microbiology, College of Biosciences, Federal University of Agriculture Abeokuta, Abeokuta-Nigeria

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Published

2026-06-23

Issue

Vol. 25 (2026)

Section

Articles