ANTIOXIDANT, ANTI-INFLAMMATORY AND HEPATOPROTECTIVE ACTIVITIES OF Citrullus LANATUS RIND ON PARACETAMOL-INDUCED HEPATOTOXIC MALE WISTAR RATS
Keywords:
Watermelon; acetaminophen; liver damage; oxidation inhibitors; swelling reducersAbstract
Hepatotoxicity is a silent killing disease which may arise due to lifestyle and drug abuse. Paracetamol (PCM) is a common drug that is regularly used with and without physician prescription. An overdose of PCM can cause hepatotoxicity. Citrullus lanatus rind (CLR) is known to possess various beneficial properties such as antioxidant and anti-inflammatory effects. This study evaluated antioxidant, anti-inflammatory and hepatoprotective activities of CLR on PCM-induced hepatotoxic Wistar rats. Thirty male Wistar rats were distributed into six groups, namely: normal saline (NS), PCM, Silymarin (SLY), 100, 200 and 400 mg/kg CLR, respectively. Rats were pre-treated with SLY, 100, 200 and 400 mg/kg CLR for fourteen days prior to a single dose of 2000 mg/kg of PCM on fifteenth day. Antioxidant and inflammatory markers were evaluated in the liver. It was observed that 400 mg/kg CLR-treated rats had significantly increased antioxidant parameters such as catalase and glutathione –s- transferase in rat liver. There were observable hepatic atrophies in liver of rats treated with PCM which were prevented in CLR-treated rats. It was seen in the liver of CLR-treated rats that there was increased level of Cyptochrome p450, but reduced levels of Interleukin -6 and Tumour Necrotic factor- α in CLR-treated rats. It may be concluded that CLR demonstrated antioxidant, anti-inflammatory and hepatoprotective effects on PCM-induced hepatotoxic rats which may be suggestive of the potential to safeguard against hepatotoxicity.
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