HEPATOPROTECTIVE EFFECTS OF ETHANOLIC EXTRACT OF TRIDAX PROCUMBENS LINN. AGAINST PARACETAMOL-INDUCED HEPATOTOXIC RATS BY INHIBITING INFLAMMATORY RESPONSE
Keywords:
Hepatotoxicity, Inflammation, Paracetamol-induced, Rats, Tridax procumbens.Abstract
ABSTRACTParacetamol (PCM) overdose has been associated with hepatotoxicity. Medicinal plants such as Tridax procumbens L. (TP) may be used to relief PCM hepatotoxicity. This study evaluated the hepatoprotective effects of TP against PCM-induced hepatotoxic rats by inhibiting inflammatory response. Twenty-four rats were used for this study, randomly distributed into six groups with four rats per group: Group A received no treatment; Group B received 300 mg/kg PCM; Group C received 50 mg/kg Silymarin; Groups D, E and F received 100, 200 and 400 mg/kg of TP respectively for 8 days. Groups C to F were treated with 300 mg/kg PCM on 9th and 10th day. The live body weight of all the rats were taken at 11th days. Blood samples were collected into plain sample bottles from all rats. Rats were sacrificed and livers were harvested, weighed and later soaked in formalin for histopathological and immunohistochemical analysis. Serum aspartate aminotransferase (AST), alanine aminotransferase (ALT) and alkaline phosphatase (ALP) were analyzed using Randox kits. Immunohistochemical techniques were used to determine Cytochrome P450 (CYT P450) and Tumour necrotic factor-α (TNF-α) antigen–antibody reactions in the hepatocytes of rat. Data were statistically analyzed with graph pad prism 5.0 using Tukey post doc test. There were decreased live body and live body-liver weight ratio, AST, ALT and ALP of rats treated with 200 and 400 mg/kg TP compared with rats treated with PCM. Reduced necrosis and inflammatory infiltrate were observed in liver of TP-treated groups. Immunohistochemical analysis showed reduced expression of inflammatory markers with CYT P450 and TNF-α in liver tissues of TP-treated rats, thus establishing the fact that TP may have hepatoprotective effects. It can therefore be inferred that TP can be incorporated into herbal preparation as a hepatoprotective medicinal plant for livestock production.
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