DISTRIBUTION AND ANTIMICROBIAL RESISTANCE PATTERNS OF Salmonella SEROVARS IN POULTRY CARCASSES IN LAGOS STATE, SOUTHWEST, NIGERIA
Keywords:
antimicrobial resistance, chicken, Nigeria, Salmonella, serovarsAbstract
Salmonellosis is a major bacterial disease causing huge economic losses in the poultry industry globally. Most of the Salmonella infections in humans results from the consumption of contaminated poultry and poultry products. This cross-sectional study determined the prevalence of non-typhoidal Salmonella, their serovars and the associated antibiotic resistance pattern in processed chicken carcasses in Lagos, Nigeria. A total of 100 dressed chicken carcasses spread across old layers (n=50) and broilers (n=50) were randomly collected in different live bird markets in Lagos, Nigeria. Culture and identification of Salmonella spp. were performed using standard bacteriological techniques followed by antimicrobial susceptibility test using the disk diffusion technique. Serotyping was performed at a Salmonella reference laboratory in Padova, Italy. A 16% Salmonella prevalence was obtained in this study spread across eight serovars (Bredeney, Wagadugu, Telelkebir, Corvallis, Chomedey, Kentucky, Nima and Hato). Five (31.25%) isolates were found in broilers while 11 (68.75%) isolates were from old layers. Of the 16 antimicrobial agents tested, seven of the positive Salmonella isolates were susceptible to all antimicrobial agents tested while out of the remaining 9 isolates, 6 were resistant to nalixidic acid (NAL), 5 showed resistance to each of spiramycin (SPR), sulphonamides (SSS) and tetracycline (TET). The presence of non-typhoidal Salmonella serovars and the antimicrobial resistant nature of some serovars impose public health challenges on the healthcare system and suggest poor hygiene practices and misuse/abuse of drugs in the poultry industry in the study location.
References
Agbaje, M., Lettini, A.A., Ojo, O. E., Longo, A., Marafin, E., Antonello, K., Zavagnin, P., Oluwasile, B.B., Omoshaba, E.O., Dipeolu, M. A. 2019. Antimicrobial resistance profiles of Salmonella serovars isolated from freshly dressed chicken meat at slaughter in Kaduna, Nigeria. Revue d’elevage Medecine Veterinaire des pays Tropicaux, 72 (4): 00-00, doi: 10.19182/remvt.31484.
Beyene T., Endalamaw E., Tolossa Y. and Feyisa A. 2015. Evaluation of rational use of veterinary drugs in Bishoftu. Central Ethiopia BMC Research Notes, 8:482.
Brisabois, A. 2001. Interet et limites des techniques de caracterisation des Salmonella. Epidemiologie et Sante Animale 39:31–42.
Clinical and Laboratory Standards Institute-CLSI 2012. Performance Standards for Antimicrobial Disk Susceptibility Tests; Approved Standard—Eleventh Edition. CLSI Document M02-A11. Clinical and Laboratory Standards Institute, Wayne, 32(1).
Fagbamila I.O., Barco L., Mancin M., Kwaga J., Ngulukun S.S., Zavagnin P., Lettini A.A., Lorenzetto M., Adbu P. A., Kabir J., Umoh J., Ricci A. and Muhammad M. 2017. Salmonella serovars and their distribution in Nigerian commercial chicken layer farms. PLoS One 12(3): 1–15.
Food and Agriculture Organization (FA0) 2006. Nigeria Poultry Sector Country Review. http://www.fao.org/3/ai352e/ai352e.pdf
Grimont, P.A.D, Grimont and F., Bouvet, P. 2000. Taxonomy of the genus Salmonella. In: Salmonella in Domestic Animals. Eds C. Wray, A. Wray. CABI Publishing, United Kingdom.
Grimont P.A.D., Weill F.X. 2007. Antigenic formulae of the Salmonella serovars, 9th Edition. Paris: WHO Collaborating Center for Reference and Research on Salmonella at Institut Pasteur in France 150 p.
Ha, T. A., Pham, T. Y. 2006. Study of Salmonella, Campylobacter, and Escherichia coli contamination in raw food available in factories, schools, and hospital canteens in Hanoi, Vietnam. Annals of the New York Academy of Science, 1081:262-265.
Hald, T., Lo Fo Wong, D. M., Aarestrup, F. M. 2007. The attribution of human infections with antimicrobial resistant Salmonella bacteria in Denmark to sources of animal origin. Foodborne Pathogen Diseases, 4: 313-326.
Jibril A.H., Okeke I.N., Dalsgaard A., Kudirkiene E., Akinlabi O.C., Bello M.B., Olsen E.J. 2020. Prevalence and risk factors of Salmonella in commercial poultry farms in Nigeria. PLoS ONE, 15(9): e0238190. https://doi.org/10.1371/journal. pone.0238190.
Kariuki, S., Revathi, G., Kiiru, J., Mengo, D. M., Mwituria, J., Muyodi, J., Munyalo, A., Teo, Y. Y., Holt, K. E., Kingsley, R. A., Dougan, G. 2010. Typhoid in Kenya is associated with a dominant multidrug-resistant Salmonella enterica serovar Typhi haplotype that is also widespread in Southeast Asia. Journal of Clinical Microbiology, 48(6): 2171–6. doi:10.1128/JCM.01983-09.
Meakins S., Fisher I.S., Berghold C, Gerner-Smidt P., Tschäpe H., Cormican M., Luzzi I., Schneider F., Wannett W., Coia J., Echeita A., Threfall E. J. 2008. Antimicrobial drug resistance in human nontyphoidal Salmonella isolates in Europe 2000-2004: a report from the enter-net international surveillance network. Microbial and Drug Resistance, 14(1):31–35.
Okeke I.N., Laxminarayan R., Bhutta Z.A., Duse A.G., Jenkins P., O'Brien T.F., Pablos-Mendez A., Klugman K.P. 2005. Antimicrobial resistance in developing countries. Part I: recent trends and current status. Lancet Infectious Diseases, 5(8):481-93. doi: 10.1016/S1473-3099(05)70189-4. PMID: 16048717.
Oluwasile B.B., Agbaje M., Ojo O.E. and Dipeolu M.A. 2014. Antibiotic usage pattern in selected poultry farms in Ogun State. Sokoto Journal of Veterinary Sciences, 12(1): 45–50.
Parveen S., Taabodi M., Schwarz J.G., Oscar T.P., Harter-Dennis J. and White D.G. 2007. Prevalence and AMR of Salmonella recovered from processed poultry. Journal of Food Protection, 70(11): 2466–2472.
Smith S., Braun S., Akintimehin F., Fesobi T., Bamidele M., Coker A., Monecke S., Ehricht R. 2016. Serogenotyping and antimicrobial susceptibility testing of Salmonella spp. isolated from retail meat samples in Lagos, Nigeria. Molecular and Cellular Probes, 30: 189-194.
Tindall B.J., Grimont P.A., Garrity G.M., Euzeby J.P. 2005. Nomenclature and taxonomy of the genus Salmonella. International Journal of Systematic and Evolutionary Microbiology, 55:521-524.
U.S. Department of Agriculture, Food Safety and Inspection Service (USDA-FSIS, 2013. Quarterly progress report on Salmonella and Campylobacter testing of selected raw meat and poultry products: preliminary results, October 2012 to December 2012. Available at: http://www.fsis.usda. gov/Science/Q4_2012_Salmonella_Testing/index.asp. Accessed 2
May 2013.
United Nation Development Programme-UNDPs 2006. Socio- Economic Impact of Avian Influenza in Nigeria, UNDP Nigeria, Abuja.
Uyttendaele M.R., Debevere J.M., Lips R.M., Neyts K.D. 1998. Prevalence of Salmonella in poultry carcasses and their products in Belgium. International Journal of Food Microbiology, 40:1–8.
Wu D., Alali W. Q., Harrison A. M., Hofacre L. C. 2014. Prevalence of Salmonella in Neck skin and bone of Chickens. Journal of Food Protection, 77 (7): 1193–1197.
