ANNONA MURICATA LINN. ETHANOLIC LEAF EXTRACT AMELIORATES REPRODUCTIVE COMPLICATIONS IN STREPTOZOTOCIN-INDUCED DIABETIC WISTAR RATS
DOI:
https://doi.org/10.51406/jnset.v18i1.2040Keywords:
Annona muricata, diabetes, streptozotocin, reproductive dysfunctionsAbstract
Diabetes mellitus is an endocrine and metabolic disorder of humans and animals characterized by hyperglycemia and low blood insulin levels or insensitivity of target organs to insulin and it’s a major health problem affecting patient’s quality of life due to its many complications. Infertility is one of the major secondary complications in diabetes. Although numerous drugs have been used for intervention studies on diabetes-induced infertility worldwide, there are currently no treatments for diabetes associated infertility in humans. This study was performed to investigate the effects of Annona muricata ethanolic leaf extract (AMELE) on fertility of male diabetic rats and levels of blood glucose. Twenty male Wistar rats (150-200g) were randomly distributed into 4 groups (n=5) treated thus: CTRL (control), DNT, DT1 and DT2 (diabetic, single intraperitoneal injection, streptozotocin, 60 mg/kg). Group DT1 and DT2 received AMELE orally at 100 mg/kg and 200 mg/kg respectively daily for fourteen days. Data were analysed using ANOVA at α0.05. The animals were sacrificed after 2 weeks via thiopental injection and testicular weights were recorded. Fasting blood glucose was determined using a digital glucometer. Sperm count, motility, viability and morphology were assessed microscopically. Testes were histologically evaluated. The results showed that oral administration of AMELE at 100 mg/kg and 200 mg/kg to diabetic male rats for fourteen days significantly decreased blood glucose level and also ameliorated diabetes-induced decreases in sperm functions in streptozotocin-induced diabetic male rats.
References
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Adeyemi, O., Komolafe, O., Adewole, O. and Obuotor, M. 2010. Histomorphological and morphometric studies of the pancreatic islet cells of diabetic rats treated with extracts of Annonamuricata. Folia Morphologica, 69:92–100.
Afifi, M., Almaghrabi, O.A. and Kadasa, N.M. 2015. Ameliorative effect of zinc oxide nanoparticles on antioxidants and sperm characteristics in streptozotocin- induced diabetic rat testes. Biomedical Research International, 2: 1–6.
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Akbarzadeh, A., Norouzian, D., Mehrabi, M.R., Jamshidi, Sh., Farhangi, A., Allah Verdi, A., Mofidian, S.M.A. and Lame Rad, B. 2007. Induction of diabetes by streptozotocin in rats. Indian Journal of Clinical Biochemistry, 22 (2): 60-64.
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Baccetti, B., Marca, L.A. and Piomboni, P. 2002. Insulin-dependent diabetes in men is associated with hypothalamo-pituitary derangement and with impairment in semen quality. Human Reproduction, 17: 2673-2677.
Butler, A.E., Janson, J., Bonner‑Weir, S., Ritzel, R., Rizza, R.A., Butler, P.C. 2003. Beta‑cell deficit and increased beta‑cell apoptosis in humans with type 2 diabetes. Diabetes, 52: 102–110.
Cavallini, G. 2006. Male idiopathic oligoasthenoteratozoospermia. Asian Journal of Andrology, 8: 143–157.
Cooke, D.W. and Plotnick, L. 2008. Type 1 diabetes mellitus in pediatrics. Pediatrics Review Journal, 29: 374–384.
De Feo, V. 1992. Medicinal and magical plants in the northern Peruvian Andes. Fitoterapia, 3: 417-440.
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Guariguata, L., Whiting, D.R., Hambleton, I., Beagley, J., Linnenkamp, U. and Shaw, J.E. 2014. Global estimates of diabetes prevalence for 2013 and projections for 2035. Diabetes Research and Clinical Practice, 103: 137–49.
Haddock, R.L. 1994: “Some medicinal plants of Guam including English and Guamanian common names.” Report Regional Tech Mtg Med Plants, Papeete, Tahiti, Nov, 1973; South Pacific Commision, Noumea, New Caledonia, 79.
Kahn, S.E., Hull, R.L. and Utzschneider, K.M. 2006. Mechanisms linking obesity to insulin resistance and type 2 diabetes. Nature, 444: 840–6.
Kameswara, R.B., Kesavulu, M.M., Guiri, R. and Apparao, C.H. 1999. Hepatic key enzyme in experimental diabetes. Journal of Ethnopharmacology, 91(1): 109-13.
Kilarkaje, N., Al‑Hussaini, H. and Al‑Bader, M.M. 2014. Diabetes‑induced DNA damage andapoptosis are associated with poly (ADP ribose) polymerase 1 inhibition in the rat testis. European Journal ofPharmacology, 737: 29–40.
Koroglu, P., Senturk, G.E., Yucel, D., Ozakpinar, O.B., Uras, F. and Arbak, S. 2015. The effect of exogenous oxytocin on streptozotocin (STZ) induced diabetic adult rat testes. Fertility and Sterility, 2: 47–54.
Maiti, R., Jana, D., Das, U.K. and Ghosh, D. 2004. Antidiabetic effect of aqueous extract of seed of Tamarindus indica in streptozotocin induced diabetic rats. Journal of ethnopharmacology, 2: 85-91.
Mors, B. 2000. Medicinal plants of Brazil. J.ournal of Natural Products, 64 (9): 1258–1259.
Oladipo, T.A., Adebiyi, A.A., Adetomiwa, A.S. Oladipo, O.O., Adeleye O.E. and Olukunle J.O. 2018. Evaluation of spermatogenic activity of Annona muricata methanolic bark extract in albino rats. Egyptian Journal of Veterinary Science, 49 (2): 83-90.
Philippe, J. and Raccah, D. 2009. Treating type 2 diabetes: how safe are current therapeutic agents? International Journal of Clinical Practice, 63: 321–332.
Rashid, K. and Sil, P.C. 2015. Curcumin ameliorates testicular damage in diabetic rats by suppressing cellular stress-mediated mitochondria and endoplasmic reticulum-dependent apoptotic death. Biochimia et Biophysica Acta, 3: 70–82.
Riserus, U., Willett, W.C. and Hu, F.B. 2009. Dietary fats and prevention of type 2 diabetes. Progress in Lipid Research, 48: 44–51.
Rupeshkumar, M., Kavitha, K. and Haldar, K. 2014. The role of herbal plants in the diabetes mellitus therapy: an overview. International Journal of Applied Pharmacology, 6: 1–3.
Sawant, R. and Dongre, P. 2014. Biochemical compositional analysis of Annonamuricata: a miracle fruit’s review. International Journal of Universal Pharmacy and Bio Sciences, 3: 82–104.
Saumya, S. and Basha, P.M. 2016. Fluoride exposure aggravates the testicular damage and sperm quality in diabetic mice: protective role of Ginseng and Banaba. Biological TraceElement Research, 2: 1–14.
Scarano, W.R., Messias, A.G., Oliva, S.U., Klinefelter, G.R. and Kempinas, W.G. 2006. Sexual behaviour, sperm quantity and quality after short-term streptozotocin-induced hyperglycaemia in rats. International Journal Andrology, 29: 482-488.
Sexton, W.J. and Jarow, J.P. 1997. Effect of diabetes mellitus upon male reproductive function. Urology Research, 49: 508-513.
Shi, D., Lia, Z., Zhenga, L., Chenb, J. and Lif, U. 2017. Diabetes associated with male reproductive system damages: onset of presentation, pathophysiological mechanisms and drug intervention. Biomedicine & pharmacotherapy, 1: 562-574.
Suneel, A., Venkatarathanamma, V., Naga, V. and Seetha, K. 2015. Antipyretic activity of Annona plants leaves on brewer’s yeast induced febrile rats. Asian Journal of Pharmaceutical and Clinical Research, 8: 210–212.
Tsounapi, P., Honda, M., Dimitriadis, F., Kawamoto, B., Hikita, K., Muraoka, K., Saito, M., Sofikitis, N. and Takenaka A. 2016. Impact of antioxidants on seminal vesicles function and fertilizing potential in diabetic rats. Asian Journal of Andrology, 2: 1–10.
Uno, U., Ogbe, H., Okolo, C., Ekaluo, U. and Akwa B. 2017. Effect of Annona muricata leaf extract on sperm toxicity induced by caffeine in albino rats. The Pharmaceutical and Chemical Journal, 4(1): 82-87.
Vasquez, M.R. 1990. Useful plants of Amazonian Portland: R. F. Standman E. R., Oliver C. N. (1991): Metal-catalyzed oxidation of proteins. Physiological consequences. Journal of Biology and Chemistry, 266: 2005-2008.
Vlad, I. and Popa A.R. 2016. Epidemiology of diabetes mellitus: a current review. Diabetes Nutrition, Metabolism and cardiovascular Diseases. 2: 433–440.
Zemjanis R. 1977. Collection and evaluation of semen in: Diagnostic and Therapeutic Techniques in Animal Reproduction. 3rd edn. William and Wilkins Company. p. 242.
Adedara, I.A., Awogbindin, I.O., Anamelechi, J.P. and Farombi, E.O. 2015. Garcinia kola seed ameliorates renal hepatic, and testicular oxidative damage in streptozotocin-induced diabetic rats. Pharmaceutical Biology, 1: 695–704.
Adewole, O. and Caxton-martins, A. 2006. Morphological changes and hypoglycemic effects of Annonamuricata linn. (Annonaceae) leaf aqueous extract on pancreatic β-cells of streptozotocin treated diabetic rats. African Journal of Biomedical Research, 9: 173–87.
Adeyemi, O., Komolafe, A., Adewole, O. and Obuotor, M. 2007. Effects of Annonamuricata (Linn) on the morphology of pancreatic islet cells of experimentally-induced diabetic wistar rats. The Internet Journal of Alternative Medicine, (5):1-8.
Adeyemi, D., Komolafe, O. and Adewole, O. 2009a. Antihyperglycaemic activities of AnnonaMuricata (Linn.).Pharmaceutical Biology, 3: 729-735.
Adeyemi, O., Komolafe, O., Adewole, O. and Obuotor, M. 2009b. Antihyperglycemic activities of Annona muricata (Linn). African Journal of Traditional Complementary Alternative Medicines, 6: 62–9.
Adeyemi, O., Komolafe, O., Adewole, O. and Obuotor, M. 2010. Histomorphological and morphometric studies of the pancreatic islet cells of diabetic rats treated with extracts of Annonamuricata. Folia Morphologica, 69:92–100.
Afifi, M., Almaghrabi, O.A. and Kadasa, N.M. 2015. Ameliorative effect of zinc oxide nanoparticles on antioxidants and sperm characteristics in streptozotocin- induced diabetic rat testes. Biomedical Research International, 2: 1–6.
Agbaje, I.M., Rogers, D.A., McVicar, C.M., McClure, N., Atkinson, A.B., Mallidis, C. and Lewis, S.E.M. 2007. Insulin dependant diabetes mellitus: implications for male reproductive function. Human Reproduction, 22: 1871–7.
Akkati, S., Sam, K.G. and Tungha, G. 2011. Emergence of promising therapies in diabetes mellitus. Journal of Clinical Pharmacology, 51: 796–804.
Akbarzadeh, A., Norouzian, D., Mehrabi, M.R., Jamshidi, Sh., Farhangi, A., Allah Verdi, A., Mofidian, S.M.A. and Lame Rad, B. 2007. Induction of diabetes by streptozotocin in rats. Indian Journal of Clinical Biochemistry, 22 (2): 60-64.
Amidu, N., Owiredu, W.K., Alidu, H., Sarpong, C., Gyasisarpong, C.K. and Quaye, L. 2013. Association between metabolic syndrome and sexual dysfunction among men with clinically diagnosed diabetes. Diabetology and Metabolic Syndrome, 5: 242–250.
Ayensu, E.S. 1981. Medicinal Plants of the West Indies. pp. 1–282, Reference Publications, Michigan.
Baccetti, B., Marca, L.A. and Piomboni, P. 2002. Insulin-dependent diabetes in men is associated with hypothalamo-pituitary derangement and with impairment in semen quality. Human Reproduction, 17: 2673-2677.
Butler, A.E., Janson, J., Bonner‑Weir, S., Ritzel, R., Rizza, R.A., Butler, P.C. 2003. Beta‑cell deficit and increased beta‑cell apoptosis in humans with type 2 diabetes. Diabetes, 52: 102–110.
Cavallini, G. 2006. Male idiopathic oligoasthenoteratozoospermia. Asian Journal of Andrology, 8: 143–157.
Cooke, D.W. and Plotnick, L. 2008. Type 1 diabetes mellitus in pediatrics. Pediatrics Review Journal, 29: 374–384.
De Feo, V. 1992. Medicinal and magical plants in the northern Peruvian Andes. Fitoterapia, 3: 417-440.
Eizirik, D.L., Colli, M.L. and Ortis, F. 2009. The role of inflammation in insulitis and beta‑cell loss in type 1 diabetes. Nature Review Endocrinology, 5: 219–26.
Ekaluo, U., Ikpeme, E., Ibiang Y. and Omordia, F. 2013. Effect of Annona muricata fruit extract on sperm toxicity induced by caffeine in albino rats. Journal of Medical Science, (13):67-71.
Freitas, J.P., Filipe, P.M., and Rodrigo, F.G. 1997. Lipid peroxidation intype 2 normolipidemic diabetic patients. Diabetes Research and Clinical Practice, 36: 71–75.
Garber, J.C., Barbee, R.W., Bielitzki, J.T., Clayton, L.A., Donovan, J.C., Hendriksen, C.F.M., Kohn, D.F., Lipman, N.S., Locke, P.A., Melcher, J., Quimby, F.W., Turner, P.V., Wood, G.A., Würbel, H. 2011. Guide for the Care and Use of Laboratory Animals, National Academy of Sciences. Published by the National Institutes of Health, National Academies Press, Washington DC, 8th Ed.
Ghanbari, E., Nejati, V., Najafi, G., Khazaei, M. and Babaei, M. 2015. Study on the effect of royal jelly on reproductive parameters in streptozotocin-induced diabetic rats. International Journal of Fertility and Sterility,1: 113–120.
Guariguata, L., Whiting, D.R., Hambleton, I., Beagley, J., Linnenkamp, U. and Shaw, J.E. 2014. Global estimates of diabetes prevalence for 2013 and projections for 2035. Diabetes Research and Clinical Practice, 103: 137–49.
Haddock, R.L. 1994: “Some medicinal plants of Guam including English and Guamanian common names.” Report Regional Tech Mtg Med Plants, Papeete, Tahiti, Nov, 1973; South Pacific Commision, Noumea, New Caledonia, 79.
Kahn, S.E., Hull, R.L. and Utzschneider, K.M. 2006. Mechanisms linking obesity to insulin resistance and type 2 diabetes. Nature, 444: 840–6.
Kameswara, R.B., Kesavulu, M.M., Guiri, R. and Apparao, C.H. 1999. Hepatic key enzyme in experimental diabetes. Journal of Ethnopharmacology, 91(1): 109-13.
Kilarkaje, N., Al‑Hussaini, H. and Al‑Bader, M.M. 2014. Diabetes‑induced DNA damage andapoptosis are associated with poly (ADP ribose) polymerase 1 inhibition in the rat testis. European Journal ofPharmacology, 737: 29–40.
Koroglu, P., Senturk, G.E., Yucel, D., Ozakpinar, O.B., Uras, F. and Arbak, S. 2015. The effect of exogenous oxytocin on streptozotocin (STZ) induced diabetic adult rat testes. Fertility and Sterility, 2: 47–54.
Maiti, R., Jana, D., Das, U.K. and Ghosh, D. 2004. Antidiabetic effect of aqueous extract of seed of Tamarindus indica in streptozotocin induced diabetic rats. Journal of ethnopharmacology, 2: 85-91.
Mors, B. 2000. Medicinal plants of Brazil. J.ournal of Natural Products, 64 (9): 1258–1259.
Oladipo, T.A., Adebiyi, A.A., Adetomiwa, A.S. Oladipo, O.O., Adeleye O.E. and Olukunle J.O. 2018. Evaluation of spermatogenic activity of Annona muricata methanolic bark extract in albino rats. Egyptian Journal of Veterinary Science, 49 (2): 83-90.
Philippe, J. and Raccah, D. 2009. Treating type 2 diabetes: how safe are current therapeutic agents? International Journal of Clinical Practice, 63: 321–332.
Rashid, K. and Sil, P.C. 2015. Curcumin ameliorates testicular damage in diabetic rats by suppressing cellular stress-mediated mitochondria and endoplasmic reticulum-dependent apoptotic death. Biochimia et Biophysica Acta, 3: 70–82.
Riserus, U., Willett, W.C. and Hu, F.B. 2009. Dietary fats and prevention of type 2 diabetes. Progress in Lipid Research, 48: 44–51.
Rupeshkumar, M., Kavitha, K. and Haldar, K. 2014. The role of herbal plants in the diabetes mellitus therapy: an overview. International Journal of Applied Pharmacology, 6: 1–3.
Sawant, R. and Dongre, P. 2014. Biochemical compositional analysis of Annonamuricata: a miracle fruit’s review. International Journal of Universal Pharmacy and Bio Sciences, 3: 82–104.
Saumya, S. and Basha, P.M. 2016. Fluoride exposure aggravates the testicular damage and sperm quality in diabetic mice: protective role of Ginseng and Banaba. Biological TraceElement Research, 2: 1–14.
Scarano, W.R., Messias, A.G., Oliva, S.U., Klinefelter, G.R. and Kempinas, W.G. 2006. Sexual behaviour, sperm quantity and quality after short-term streptozotocin-induced hyperglycaemia in rats. International Journal Andrology, 29: 482-488.
Sexton, W.J. and Jarow, J.P. 1997. Effect of diabetes mellitus upon male reproductive function. Urology Research, 49: 508-513.
Shi, D., Lia, Z., Zhenga, L., Chenb, J. and Lif, U. 2017. Diabetes associated with male reproductive system damages: onset of presentation, pathophysiological mechanisms and drug intervention. Biomedicine & pharmacotherapy, 1: 562-574.
Suneel, A., Venkatarathanamma, V., Naga, V. and Seetha, K. 2015. Antipyretic activity of Annona plants leaves on brewer’s yeast induced febrile rats. Asian Journal of Pharmaceutical and Clinical Research, 8: 210–212.
Tsounapi, P., Honda, M., Dimitriadis, F., Kawamoto, B., Hikita, K., Muraoka, K., Saito, M., Sofikitis, N. and Takenaka A. 2016. Impact of antioxidants on seminal vesicles function and fertilizing potential in diabetic rats. Asian Journal of Andrology, 2: 1–10.
Uno, U., Ogbe, H., Okolo, C., Ekaluo, U. and Akwa B. 2017. Effect of Annona muricata leaf extract on sperm toxicity induced by caffeine in albino rats. The Pharmaceutical and Chemical Journal, 4(1): 82-87.
Vasquez, M.R. 1990. Useful plants of Amazonian Portland: R. F. Standman E. R., Oliver C. N. (1991): Metal-catalyzed oxidation of proteins. Physiological consequences. Journal of Biology and Chemistry, 266: 2005-2008.
Vlad, I. and Popa A.R. 2016. Epidemiology of diabetes mellitus: a current review. Diabetes Nutrition, Metabolism and cardiovascular Diseases. 2: 433–440.
Zemjanis R. 1977. Collection and evaluation of semen in: Diagnostic and Therapeutic Techniques in Animal Reproduction. 3rd edn. William and Wilkins Company. p. 242.
