BIOLOGICAL CONTROL OF FUSARIUM WILT OF CUCUMBER (Cucumis sativus) BY ANTAGONISTIC LACTIC ACID BACTERIA ISOLATED FROM RHIZOSPHERE OF FIVE MEDICINAL PLANTS
DOI:
https://doi.org/10.51406/jnset.v18i1.2035Keywords:
Biocontrol, Cucumber, Fusarium wilt, Lactic acid bacteria, Medicinal plants, RhizosphereAbstract
Fusarium wilt is one of the important diseases of cucumber and causes economic loss to farmers. The present study was undertaken to evaluate the potential of rhizosphere lactic acid bacteria as biocontrol agents of Fusarium wilt of cucumber. Lactic acid bacteria (LAB) were isolated and identified from the rhizosphere of five medicinal plants. The in-vitro antagonistic activity of LAB strains on F. oxysporum f.sp. cucumerinum was evaluated by dual culture method. The screen house experiment was then conducted to assess the effect of antagonistic LAB isolates on Fusarium wilt disease incidence in cucumber plants. The antagonistic LAB strains were further characterized using 16S rRNA gene sequencing technique. The total LAB counts of rhizospheric soil samples ranged from 7.0×105 cfu/g to 15.0×105 cfu/g. The LAB isolates were identified as strains of Lactobacillus acidophilus (21.4%), L. plantarum (35.7%), L. fermentum (28.6%), L. alimentarius (7.1%) and L. brevis (7.1%). Treatment of cucumber seeds with antagonistic LAB strains significantly reduced Fusarium wilt of cucumber incidence from 95% to 48%. Lactobacillus fermentum isolated from the rhizosphere of A. indica exhibited strong disease suppression (49.5%). The study therefore revealed that the rhizospheric-LAB could be applied to reduce the manifestation of Fusarium wilt in cucumber.
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Yanagida, F., Chen, Y. and Shinohara, T. 2005. Isolation and characterization of lactic acid bacteria from soils in vineyards. The Journal of General and Applied Microbiology. 51(5): 313 – 318.
Ahmed, G.A. 2010. Controlling of Fusarium wilt of cucumber by antagonistic bacteria. Journal of Life Sciences. 4(7): 16 – 21.
Alwathnani, H.A. and Perveen, K. 2012. Biological control of Fusarium wilt of tomato by antagonist fungi and cyanobacteria. African Journal of Biotechnology. 11(5): 1100 – 1105.
Chehri, K., Abbasi, S., Reddy, K.R.N. and Salleh, B. 2010. Occurrence and pathogenicity of various pathogenic fungi on cucurbits from Kermanshah province, Iran. African Journal of Microbiology Research. 4: 115 - 122.
Chen, Y.S., Yanagida, F. and Shinohara, I. 2005. Isolation and identification of lactic acid bacteria from soil using an enrichment procedure. Letters in Applied Microbiology. 40(3): 195–200.
Dinesh, R., Anandaraj, M., Kumar, A., Bini, Y.K., Subila, K.P. and Aravind, R. 2015. Isolation, characterization, and evaluation of multi-trait plant growth promoting rhizobacteria for their growth promoting and disease suppressing effects on ginger. Microbiological Research. 173: 34 – 43.
Ekundayo, F.O. 2014. Isolation and identification of lactic acid bacteria from rhizosphere soils of three fruit trees, fish and ogi. International Journal of Current Microbiology and Applied Sciences. 3(3): 991-998.
El-Mabrok, A.S.W., Hassan, Z. and Mokhtar, A.M. 2012. Screening of lactic acid bacteria as biocontrol against Colletotrichum capsici on Chilli Bangi. Research Journal of Applied Sciences. 7(9 - 12): 466 – 473.
Farrag, E.S.H. and Moharam, M.H.A. 2012. Pathogenic fungi transmitted through seeds and safely elimination by application of peppermint extract and oil. Notulae Scientia Biologicae. 4(3): 83- 91.
Fhoula, I., Najjari, A., Turki, Y., Jaballah, S., Boudabous, A. and Ouzari, H. 2013. Diversity and antimicrobial properties of lactic acid bacteria isolated from rhizosphere of olive trees and desert truffles of tomato. Biomedical Research International. 13: 1-14.
Fravel, D.R., Deahl, K.L. and Stommel, J.R. 2005. Compatibility of the biocontrol fungus Fusarium oxysporum strain CS-20 with selected fungicides. Biological Control. 34(2): 165 -169.
Hamed, H.A., Moustafa, Y.A. and Abdel-Aziz, S.M. 2011. In vivo efficacy of lactic acid bacteria in biological control against Fusarium oxysporum for protection of tomato plant. Life Science Journal. 8(4): 462-468.
Idris, H.A., Labuschagne, N. and Korsten, L. 2007. Screening rhizobacteria for biological control of Fusarium root and crown rot of sorghum in Ethiopia. Biological Control. 40(1): 97-106.
Jiang, H., Dong, H., Zhang, G., Yu, B., Chapman, L.R. and Fields, M.W. 2006. Microbial Diversity in Water and Sediment of Lake Chaka, an Athalassohaline Lake in Northwestern China. Applied and Environmental Microbiology. 72(6): 3832-3845.
Khanzada, K.A., Rajput, M.A., Shah, G.S., Lodhi, A.M. and Mehboob, F. 2002. Effect of seed dressing fungicides for the control of seed-borne mycoflora of wheat. Asian Journal of Plant Sciences. 1: 441 – 444.
Mahfooz, S., Maurya, D.K., Srivastava, A.K., Kumar, S. and Arora, D.K. 2011. A comparative in-silico analysis on frequency and distribution of microsatellites in coding regions of three formae speciales of Fusarium oxysporum and development of EST-SSR markers for polymorphism studies. FEMS Microbiology Letters. 328(1): 54 – 60.
Maji, S. and Chakrabartty, P.K. 2014. Biocontrol of bacterial wilt of tomato caused by Ralstonia solanacearum by isolates of plant growth-promoting rhizobacteria. Australian Journal of Crop Science. 8(2): 208 – 214.
Nawangsih, A.A. and Purba, F. 2013. Isolation of fluorescent Pseudomonads, heat tolerant and chitinolytic bacteria in banana rhizosphere with antagonistic activities against Fusarium oxysporum f. sp. cubense in vitro and molecular identification of selected isolates. Journal of the International Society for Southeast Asian Agricultural Sciences. 19(2): 30 - 40.
Oloyede, A., Ajijola W. and Albert, O. 2017. Characterization and evaluation of rhizobacteria isolated from selected medicinal plants for plant growth promoting and antagonism of Fusarium oxysporum f.sp cucumerinum. Proceedings of the Maiden Bioscience Annual Conference, pp 69-76.
Saha, D., Purkayastha, G.D., Ghosh, A., Isha, M. and Saha, A. 2012. Isolation and characterization of two new Bacillus subtilis strains from the rhizosphere of egg plant as potential biocontrol agents. Journal of Plant Pathology. 94(1): 109 – 118.
Sathe, S.J., Nawani, N.N., Dhakephalkar, P.K. and Kapadnis, B.P. 2007. Antifungal lactic acid bacteria with potential to prolong shelf-life of fresh vegetables. Journal of Applied Microbiology. 103: 2622 – 2628.
Shanthi, A.T. and Vittal, R.R. 2013. Biocontrol potentials of plant growth-promoting rhizobacteria against Fusarium wilt disease of Cucurbit. ESci Journal of Plant Pathology. 2(3): 155 – 161.
Trias, R., Baneras, L., Montesinos, E. and Badosa, E. 2008. Lactic acid bacteria from fresh fruits and vegetables as biocontrol agents of phytopathogenic bacteria and fungi. International Microbiology. 11(4): 231 – 236.
Wang, H., Yan, H., Shin, J., Huang, L., Zhang, H.P. and Qi, W. 2011. Activity against plant pathogenic fungi of Lactobacillus plantarum IMAU10014 isolated from Xinjiang Koumiss in China. Annals of Microbiology: 61(4): 879 – 885.
Wilcox, G. I., Offor, U. S. and Omojola, J. T. 2015. Profitability of cucumber (Cucumis sativa L.) production in Tai local government area of Rivers State, Nigeria. Journal of Advanced Studies in Agricultural, Biological and Environmental Sciences. 2(3): 1 – 6.
Xue, Q. Y., Chen, Y., Li, S. M., Chen, L. F., Ding, G. C., Guo, D. W. and Guo, J. H. 2009. Evaluation of the strains of Acinetobacter and Enterobacter as potential biocontrol agents against Ralstonia wilt of tomato. Biological Control. 48 (3): 252-258.
Yanagida, F., Chen, Y. and Shinohara, T. 2005. Isolation and characterization of lactic acid bacteria from soils in vineyards. The Journal of General and Applied Microbiology. 51(5): 313 – 318.
