ANTIBACTERIAL ACTIVITIES OF HONEY FROM DIFFERENT LOCATIONS IN THE TROPICAL RAIN FOREST BELT OF NIGERIA, ON SELECTED BACTERIAL ISOLATES
DOI:
https://doi.org/10.51406/jnset.v10i2.1405Keywords:
Agar, Antibacterial activity, E. coli, Honey, Staphylococcus aureusAbstract
Honey has been used for various purposes including treatment of diseases. This study was carried out to determine antibacterial activities of honey samples obtained from different locations in the tropical Rainforest Belt of Nigeria (Abeokuta, Aiyetoro, Ajebo, and Saki) against reference bacterial isolates: Escherichia coli (E.coli) (ATCC 25922) and Staphylococcus aureus (ATCC 25923), using agar well diffusion technique. Staphylococcus aureus showed sensitivity to all honey samples used with the zone of inhibition ranging between 1.9 and 2.6mm. Escherichia coli was resistant to all concentrations of honey samples used. However, the present study did not explore the possible causative agent(s) involved in the antibacterial activity of the honey used.
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References
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Al-Somal, N., Coley, K.E., Molan, P.C., Hancock, B.M. 1994. Susceptibility of Helicobacter pylori to the Antibacterial Activity of Manuka Honey. Rus. Med. J., 87: 9–12.
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Molan, P.C. 1992a. The Antibacterial Activity of Honey. 1. The Nature of the Antibacterial Activity. Bee World, 73(1): 5-28.
Molan, P. C. 1992. The Antibacterial Activity of Honey. Variation in the Potency of the Antibacterial Activity. Bee World, 73: 59-76.
Molan, P.C. 2006. The evidence supporting the use of honey as a wound dressing. Int. J. Low Extrem Wound., 5: 40-54.
Molan, P.C., Betts, J. 2000. Using Honey Dressing. The Practical Considerations. Nursing Times, 96(49): 36-37.
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Olawuyi, A.K., Fasina, F.O., Ardzard, S., Akano, G.F., Agada, G.O., Gado, D.A. 2010. Antimicrobial activities of honey from different geographical locations on gram negative and positive organisms. Nig. Vet. J., 31(2): 103-114.
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Radwan, S. A., El-Essawy, M., Sarhan M. M. 1984. Experimental evidence for the occurrence in honey of specific substances active against microorganisms. Zentral Mikrobiol, 39: 249-255.
Subrahmanyan, M. 1991. Topical Application of Honey in the Treatment of Burns. Br. J. Surg., 78: 497-498.
Nuriza-Tumin, N., Arsyiah A. H., Shahjahan , M., Noor Izani N. J., Munavvar A . S., Abdul, H. K., Mohsin, J.S.S. 2005. Antibacterial activity of local Malaysian honey. Malay. J. Pharm. Sci., 3(2): 1–10.
White, J. W., Riethof, M.L., Kushnir, I. 1960. Composition of Honey. VI. The effect of storage on carbohydrates, acidity and diastase content. J. Food Sci. 26(1): 63-71.
Wilkinson, J.M., Cavanagh, H.M. 2005. Antibacterial activity of 13 honeys against Escherichia coli and Pseudomonas aeruginosa. J. Med. Food, 8: 100-103.
Willix, D. J., Molan, P. C., Harfoot, C. J. 1992. A composition of the sensitivity of wound infecting species of bacteria to the antimicrobial activity of Manuka honey and other honey. J. Appl. Bacteriol., 73: 388-394.
Woods, G., Washington, J.A. 1995. Antimicrobial Susceptibility Test dilution and disk diffusion methods. Manual of Clinical Microbiology; 6: 1327-1332.
Zumla, A., Lulat, A. 1989. Honey - a Remedy Rediscovered. J. Royal Soc. Med., 82: 384-385.
Agbaje, O.E., Ogunsanya, T., Aiwerioba, R.I.O. 2006. Conventional use of honey as antibacterial agent. Ann. Afr. Med., 5(2): 78-81.
Allen, K.L., Molan, P.C., Reid, G.M. 1991. A Survey of the Antibacterial Activity of some New Zealand Honeys. J. Pharm. Pharmacol., 43(12): 817-22.
Al-Somal, N., Coley, K.E., Molan, P.C., Hancock, B.M. 1994. Susceptibility of Helicobacter pylori to the Antibacterial Activity of Manuka Honey. Rus. Med. J., 87: 9–12.
Armon, P.J. 1980. The Use of Honey in the Treatment of Infected Wounds. Trop. Doct., 10(2): 91.
Bogdanov, S. 1984. Characterization of antimicrobial substances in honey. Ledensm Wiss. Technol., 17: 74-76.
Cortopassi-Laurino, M., Gelli, D.S. 1991. Analse pollinique, proprieties physico-chimiques et action antibacterienne des miels d’abeilles africanisees. A. mellifera et de Meliponines du Bresil. Apilogue, 22: 61-73.
Efem, S.E. 1988. Clinical Observations on the Wound Healing Properties of Honey. Br. J. Surg., 75(7): 679-81.
Jeddar, A., Kharsany, A., Ramsaroop, V.G., Bhamjee, A., Haffejee, I.E., Moosa, A. 1985. The antibacterial action of honey. S. Afr. Med. J., 67: 257-258.
Haffejee, I. E., Moosa, A. 1985. Honey in the Treatment of Infantile Gastroenteritis . Br. Med. J., 290: 1866-1867.
Leveen, H.H., Falk, G., Bore, K.B. 1973. Chemical acidification of wounds; an adjuvant to healing and the unfavourable action of alkalinity by ammonia. Ann. Surg., 187: 745 - 53.
Lusby, P.E., Coombes, A.L., Wilkinson, J.M. 2005. Bactericidal activity of different honeys against pathogenic bacteria. Arch Med Res., 36: 464-467.
Majno, G. 1975. The Healing Hand, Man and Wound in the Ancient World. Massachusetts: Harvard University Press Cambridge.
Melissa, A. M., Olga, I. P.Z., Randy, W. W. 2004. Growth inhibition of food borne pathogens and food spoilage organisms by select raw honeys. Intl. J. Food Microbiol., 97: 1–8.
Molan, P.C. 1992a. The Antibacterial Activity of Honey. 1. The Nature of the Antibacterial Activity. Bee World, 73(1): 5-28.
Molan, P. C. 1992. The Antibacterial Activity of Honey. Variation in the Potency of the Antibacterial Activity. Bee World, 73: 59-76.
Molan, P.C. 2006. The evidence supporting the use of honey as a wound dressing. Int. J. Low Extrem Wound., 5: 40-54.
Molan, P.C., Betts, J. 2000. Using Honey Dressing. The Practical Considerations. Nursing Times, 96(49): 36-37.
Mundo, M.A., Padilla-Zakour, O.I., Worobo, R.W. 2004. Growth inhibition of foodborne pathogens and food spoilage organisms by select raw honeys. Int. J. Food Microbiol., 97: 1-8.
Olawuyi, A.K., Fasina, F.O., Ardzard, S., Akano, G.F., Agada, G.O., Gado, D.A. 2010. Antimicrobial activities of honey from different geographical locations on gram negative and positive organisms. Nig. Vet. J., 31(2): 103-114.
Omafuvbe, B.O., Akanbi, O.O. 2009. Microbiological and physico-chemical properties of some commercial Nigerian honey. Afr. J. Microbiol. Res., 3(12): 891-896.
Postmes, T.J., Bosch, M.M.C., Dutrieux, R., Van Baare, J., Hoekstra, M.J. 1997. Speeding up the Healing of Burns with Honey. An Experimental Study with Histological Assessment of Wound Biopsies. In: Mizrahi A, Lensky Y, editors. Bee Products: Properties, Applications and Apitherapy. New York: Plenum Press, 27-37.
Radwan, S. A., El-Essawy, M., Sarhan M. M. 1984. Experimental evidence for the occurrence in honey of specific substances active against microorganisms. Zentral Mikrobiol, 39: 249-255.
Subrahmanyan, M. 1991. Topical Application of Honey in the Treatment of Burns. Br. J. Surg., 78: 497-498.
Nuriza-Tumin, N., Arsyiah A. H., Shahjahan , M., Noor Izani N. J., Munavvar A . S., Abdul, H. K., Mohsin, J.S.S. 2005. Antibacterial activity of local Malaysian honey. Malay. J. Pharm. Sci., 3(2): 1–10.
White, J. W., Riethof, M.L., Kushnir, I. 1960. Composition of Honey. VI. The effect of storage on carbohydrates, acidity and diastase content. J. Food Sci. 26(1): 63-71.
Wilkinson, J.M., Cavanagh, H.M. 2005. Antibacterial activity of 13 honeys against Escherichia coli and Pseudomonas aeruginosa. J. Med. Food, 8: 100-103.
Willix, D. J., Molan, P. C., Harfoot, C. J. 1992. A composition of the sensitivity of wound infecting species of bacteria to the antimicrobial activity of Manuka honey and other honey. J. Appl. Bacteriol., 73: 388-394.
Woods, G., Washington, J.A. 1995. Antimicrobial Susceptibility Test dilution and disk diffusion methods. Manual of Clinical Microbiology; 6: 1327-1332.
Zumla, A., Lulat, A. 1989. Honey - a Remedy Rediscovered. J. Royal Soc. Med., 82: 384-385.
