PLASMA LIPID PROFILE AND SOME BIOCHEMICAL INDICES IN DOMESTICATED GREATER CANE RAT (Thryonomys swinderianus temminck)
DOI:
https://doi.org/10.51406/jnset.v10i1.1341Keywords:
Biochemical parameters, Cane rat, Domestication, Lipid profile.Abstract
Some serum biochemical parameters and lipid profile indices were determined in eight male adult greater cane rats Thryonomys swinderianus Temminck, raised under intensive management system. Activities of alanine aminotransferase (ALT), aspartate aminotransferase (AST) and alkaline phosphatase (ALP) in plasma were assayed spectrophotometrically, following venopuncture-blood collection and subsequent separation of plasma. Glucose, total protein, albumin and bilirubin as well as lipid profile indices such as total cholesterol, HDL-cholesterol, LDL-cholesterol, VLDL-cholesterol, triglycerides and phospholipids concentration were also determined. In comparison with data from previous studies on wild and captive cane rats, our results indicate lower lipid profile indices and total protein. Activities of hepatic enzymes (AST and ALT) were also lower, while plasma glucose concentration was higher in this third generation greater cane rats. It is concluded that certain aspects of management system in domestication may have a profound influence on biochemical parameters most especially the lower LDL-cholesterol that reduces the risk of atherosclerosis (cardiac disease). This study, therefore offers baseline data for third generation intensively raised greater cane rats.
Â
References
Adu, E. K, Otsyina, R.H., Agyei A. D. 2005: The efficacy of different dose levels of albendazole for reducing fecal worm egg count in naturally infected captive grasscutter (Thryonomys swinderianus, Temminck). Livestock Research and Rural Development, 17(11): 1-6.
Ajayi, S.S. 1994: Ensuring sustainable management of wildlife resources: The case of Africa: FAO Forestry Paper No. 122, Rome 1994. 133 – 140.
Alarape, A.A. 2002: Cane rat (Thryonomys swinderianus) production – An effective source of income for the household. OBECHE, 24: 2 – 6.
Allain, C.C., Poon, L.S., Clau, C.S.G, Richmond, W., Fu, P.D. 1974: Enzymatic determination of total serum cholesterol. Clin. Chem., 20: 470 – 578
Asibey, E.O.A., Eyeson K. K. 1975: Additional information on the importance of wildlife animals as food source in Africa south of the Sahara. Bongo Journal of the Ghana Wildlife Society 1: 13-17
Awah, J. N., Nottidge H. O. 1998: Serum biochemical parameters in clinically healthy dogs in Ibadan. Trop. Vet., 16: 123.
Barham, D., Trinder, P. 1972: Procedure for Glucose GOD-PAP assay without deproteinization. Analyst, 97: 142
Bartels, H., Bohmers, M. 1972: Colorimetric method of Creatinine determination. Clin. Chem. Acta, 37: 193
Buccolo, G., David, H. 1973: Quantitative determination of serum triacylglycerol by the use of enzymes. Clin. Chem. 19: 476 – 482
Bush, B. M. 1991: Interpretation of Laboratory Results for Small Animal Clinicians. Blackwell scientific publication, London
Byanet, O., Adamu, S., Salami, S.O., Obadiah, H.I. 2008: Haematological and plasma biochemical parameters of the young Grasscutter (Thryonomys swinderianus) reared in Northern Nigeria. Journal of cell and Animal Biology, 2(10): 177 – 181
Coles, E. H. 1986: Veterinary Clinical Pathology. 4th ed. W.B. Saunders Co., Philadelphia, U.S.A.
Folch, J., Lees, M., Sloane S.G.H. 1957: A simple method for the isolation and purification of total lipids from animal tissues. J. Biol. Chem., 226: 497 – 509
Gidez, L. T, Miller, G.H, Burnstein M., Slagle, S., Eder, H.A. 1982: Separation and quantitation of sub-classes of human high density lipoproteins by a simple precipitation procedure. J. Lipid Res., 23: 1206 – 1223.
Ginsberg, H.N. 1994: Lipoprotein metabolism and its relationship to atherosclerosis. Med Clin North Am., 76: 1-20
Law, M.R. 1999: Lowering heart disease risk with cholesterol reduction: evidence from observational studies and clinical trials. Eur. Heart J suppl., 1: 53 – 58
Ogunsanmi A. O., Akpavie S.O., Anosa V.O. 1994: Serum biochemical changes in West African Dwarf sheep experimentally infected with Trypanosoma brucei. Rev. Elev. Med. Vet. Pays. Trop. 47(2): 195
Ogunsanmi A.O., Ozegbe P.C., Ogunjobi O., Taiwo V.O., Adu J.O. 2002: Haematological,plasma biochemistry and whole blood minerals of the captive adult African grasscutter (Thryonomis swinderianus Temminck). Trop. Vet., 20(1): 27
Ogunrinade A, Fajimi J, Adenike A. 1981: Biochemical indices in the White Fulani (Zebu) cattle in Nigeria. Rev. Elev. Med. Vet. Pays. Trop., 34(4): 41.
Ononogbu, I.C., Lewis, B. 1976: Lipoprotein fractionation by a precipitation method - A simple quantitative procedure. Clin. Chem Acta. 71: 397 – 402
Opara, M. N., Ike, K. A., Okoli, I. C. 2006: Haematology and Plasma Biochemistry of the Wild Adult African Grasscutter (Thryonomis swinderianus, Temminck). The Journal of American Science; 2(2): 17-22
Opara, M.N. 2010: The grasscutter : A livestock of tomorrow. Res. J. For., 4: 119-135.
Reitman, S., Frankel, S. 1957: A colorimetric method for the determination of serum glutamic oxaloacetic and glutamic pyruvic transaminases. Am. J. Clin. Path., 28: 56
Rose, H.G., Oklander, M. 1965: Improved procedure for the extraction of lipids from human erythrocytes. J. Lipids Res., 6: 428 – 431
Sandkamp, M., Funlce, H., Schulter, M., Kahlar, E., Assman, G. 1990: Lipoprotein is an independent risk factor for myocardial infarction at a young age. Clin. Chem., 36: 20 – 23.
Stewart, J.C.M. 1979: Colorimetric determination of phospholipids with ammonium ferrothiocyanate. Anal. Biochem. 104: 10 – 14
Stein, O. and Stein, Y. 1999. Arthroprotective mechanisms of HDL. Arterosclerosis 144: 285 – 303.
Stephens, B.H., Pavla, M.K., Maria, T.C., Martha, A.H. 2008: Haematology and plasma chemistry reference intervals for mature laboratory Pine Voles (Microtus pinetorum) as determined by using the Non-parametric rank percentile method. Journal of the American Association for laboratory Animal Science, 47(4): 35 – 40.
Sviridiv, D. 1999. Intracellular cholesterol trafficking. Histol. Histopathol., 14: 305 – 319.
Tietz, N.W. 1995: Clinical guide to laboratory texts. 3rd edition. W.B Saunder company Philadelphia PA. 518 - 519.
