BACTERIAL QUALITY AND CYTOTOXICITY SCREENING OF FRESH VEGETABLES IRRIGATED WITH POLLUTED WATERS
DOI:
https://doi.org/10.51406/jnset.v10i1.1287Keywords:
Mutagenic, Bacterial quality, Allium cepa, Chromosome aberration, and Heavy metals.Abstract
The mutagenic effects of raw vegetables irrigated with polluted water and their bacterial quality were studied. Two vegetables, lettuce (Lactuca sativa) and fluted pumpkin (Telfaria occidentalis) were planted in sterile soil and irrigated with sewage-polluted stream, rain, tap and well waters, and harvested. The presence of pathogenic bacteria on the vegetable leaf surfaces was determined. The Allium cepa assay was then used to evaluate the genetic and acute effects of the vegetable leaf extracts. The heavy metal concentrations of the vegetables were determined using atomic absorption spectrophotometry. Pathogenic bacteria isolated were Escherichia coli, Staphylococcus aureus, Salmonella paratyphii, Shigella dysenteriae, Klebsiella pneumoniae, Aeromonas hydrophila and Enterobacter aerogenes. In A. cepa assay, none of the treatments induced chromosome aberration at the tested concentrations, but retardation of growth and suppression of mitotic activity occurred. The concentrations of heavy metals in the vegetables were lead (0.261-0.531mg/kg), zinc (0.142-1.618mg/kg), cadmium (0.00-0.13mg/kg), copper (0.021-0.057mg/kg), iron (0.711-1.122mg/kg) and chromium (0.00-0.14mg/kg). This study shows that irrigation waters could have effects on the quality of edible vegetables.
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References
Bakare, A.A., Mosuro, A.A., Osibanjo, O. 1999. Cytotoxic effects of landfill leachate on Allium cepa (L). Bioscience Research Communications, 11(1): 1-13.
Bakare, A.A., Mosuro, A.A., Osibanjo, O. 2000. Effect of simulated leachate on chromosomes and mitosis in roots of
Allium cepa (L). Journal of Environmental Biology, 21(3): 263-271.
Bakare, A.A., Adekunle, A. 2001. The potential mutagenic effect of the leachates of rural solid waste landfill on Allium cepa (L). Ethiopian Journal of Science, 24(2): 283-291.
Bakare, A.A., Lateef, A., Amuda, O.S., Afolabi, O.R. 2003. The aquatic toxicity and characterization of chemical and microbiological constituents of water samples from Oba river, Odo-Oba, Nigeria. Asian Journal of Microbiology Biotech. Environmental Science, 5(1): 11-17.
Codex Alimentarius Commission. 1992. Joint FAO/WHO food standards programme, Codex Alimentarius. Pp. 106.
El-Shahaby, O.A., Abdel Migid, H.M., Soliman, M.I., Mashaly, I.A. 2003. Genotoxicity screening of industrial waste water using the Allium cepa chromosome aberration assay. Pakistan Journal of Biological Science, 6(1): 23-28.
Fiskesjo, G. 1985. The Allium tests as a standard in environmental monitoring. Hereditas, 102: 99-112.
Holt, J.G., Krieg, N.R., Sneath, P.H.A., Staley, J.T., Williams, S.T. 1994. Bergey’s Manual of Determinative Bacteriology, 9th edition. Baltimore, M. D. Williams and Wilkins (eds.).
Itanna, F. 2002. Metals in leafy vegetables grown in Addis Ababa and toxicological implications. Ethiop. Journal of Health Development, 16(3): 295-302.
Li, Y., Brackett, R.E., Chen, J., Beuchat, L.R. 2001. Survival and growth of Escherichia coli 0157:H7 inoculated onto cut lettuce before and after heating in chlorinated water, followed by storage at 5oC and 15oC. Journal of Food Protection, 64(3): 305-309.
