RANDOM AMPLIFIED POLYMORHIC DNA TYPING OF MULTIDRUG-RESISTANT CLINICAL AND ENVIRONMENTAL Pseudomonas aeruginosa STRAINS FROM ABEOKUTA, NIGERIA.
DOI:
https://doi.org/10.51406/jnset.v12i1.1272Keywords:
Pseudomonas aeruginosa, multidrug resistance, genetic relatedness, water quality, RAPD-PCR.Abstract
Pseudomonas aeruginosa, a multidrug-resistant organism is responsible for most opportunistic infections. Genetic relatedness between clinical and environmental strains has always been limited to hospital settings. This study utilized RAPD-PCR typing method to evaluate genetic relatedness between multidrug-resistant P. aeruginosa strains from diverse water samples (bottled water, tap water, sachet water, well water, hospital storage tank, and swimming pool water) and clinical strains (wound, blood, urine, eye and ear swab) collected from different locations in Abeokuta, Nigeria.   Polymorphic DNA bands with sizes ranging between 250 and 3000bp were generated from both clinical and environmental strains. Within each population, both clinical and environmental strains were divided phylogenetically into two groups of Pc1 and Pc2 at 55% and Ps1 and Ps2 at 70% respectively.   Genetic similarities between clinical and environmental strains yielded a total of 7 unique fingerprints.  Cluster 2 (51.2%) had the largest number of strains in which strains from wound, blood and ear, clustered with strains from hospital storage tank, tap water, swimming pool water, sachet water and well water.   These fingerprints proof genetic relatedness between clinical and environmental strains in Abeokuta, southwest Nigeria which is of public health significance, particularly, for immunocompromised
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References
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Akanji, B.O., Ajele, J.O., Onasanya, A., Oyelakin, O. 2011. Genetic fingerprinting of Pseudomonas aeruginosa involved in nosocomia infection as reveal by RAPD-PCR markers. Biotechnology 2:1-8.
Andualem, B, 2012. The Isolation Rate of Pseudomonas aeruginosa Opportunistic Pathogen and their Antimicrobial Responses in HIV-1 Positive and Negative Diarrhoea Patients at North-West Part of Ethiopia. Journal of AIDS and Clinical Research 3:4.
Asboe, D., Gant, V., Aucken, H.M., Moore, D.A., Umasankar, S., Bingham, J.S., Kaufmann M.E., Pitt, T.L. 1998. Persistence of Pseudomonas aeruginosa strains in Respiratory Infection in AIDS Patients. AIDS 12 (14): 1771-1775.
Bogiel, T., Gospodarek, E. 2010. PCR-RAPD typing of carbapenem-resistant Pseudomonas aeruginosa strains. Medycyna Doswiadczalna I Mikrobiologia 62(3): 211-219.
Cholley, P., Thouverez, M., Floret, N., Bertrand, X., Talon, D. 2008. The role of water fittings in intensive care rooms as reservoirs for the colonization of patients with Pseudomonas aeruginosa. Intensive Care Medicine 34(8): 1428–1433.
Deligianni, E., Pattison, S., Berrar, D., Ternan, N.G., Haylock, R.W, Moore, J.E., Elborn, S.J., Dooley, J.S.G. 2010. Pseudomonas aeruginosa Cystic Fibrosis isolates of similar RAPD genotype exhibit diversity in biofilm forming ability in vitro. BMC Microbiology 10:38Â doi: 10.1186/1471-2180-10-38.
El-Bialy, A.A., El-Shennawy, G.A., Mosaad, A.A., Bendary, L.A. 2008. Phenotyping and genotyping of Pseudomonas aeruginosa urine isolates in Zagazig University Hospitals. Egyptian Journal of Medical Microbiology (17) 4: 615-626.
Fazzeli, H., Akbari, R., Moghim, S., Narimani, T., Arabestani M.R., Ghoddousi A.R 2012. Pseudomonas aeruginosa infections in patients, hospital means, and personnel’s specimens. Journal of Research in Medical Sciences 4: 332-336.
Fichtenbaum, C.J., Woeltje K.F., Powderly W.G. 1994. Serious Pseudomonas aeruginosa infections in patients infected with Human Immunodeficiency Virus: a case control study. Clinical Infectious Disease 19: 417-422.
Finnan, S., Morrissey, J.P., O’Gara, F., Boyd, E.F. 2004. Genome diversity of Pseudomonas aeruginosa isolates from cystic fibrosis patients and the hospital environment. Journal of Clinical Microbiology 42 (12): 5783- 5792.
Gad, G.F., El-Domany, R.A., Zaki, S., Ashour, H.M. 2007. Characterization of Pseudomonas aeruginosa isolated from clinical and environmental samples in Minia, Egypt: prevalence, antibiogram and resistance mechanisms. Journal of Antimicrobial Chemotherapy 60: 1010-1017.
Gawish, A.A., Mohammed, N.A., El-Shennawy, G.A., Mohammed, H.A. 2013. An investigation of type 3 secretion toxins encoding-genes of Pseudomonas aeruginosa isolates in a University Hospital in Egypt. Journal of Microbiology and Infectious Diseases 3(3): 116-122.
Jako, E., Ari, E., Ittzes, P., Horvarth, A. and Podani, J. 2009. BOOL-AN: A method for comparative sequence analysis and phylogenetic reconstruction. Molecular and Phylogenetic Evolution 52:887-897.
Jiun-Ling, W., Mei-Ling C., Yusen E. L., Shan-Chwen C., Yee-Chun, C. 2009. Association between contaminated faucets and colonization or infection by nonfermenting gram-negative bacteria in intensive care units in Taiwan. Journal of Clinical Microbiology 47(10):3226.
Kidd, T.J., Grimwood, K., Ramsay, K.A., Rainey, P.B., Bell, S.C. 2011. Comparison of Three Molecular Techniques for Typing Pseudomonas aeruginosa Isolates in sputum samples from patients with cystic fibrosis. Journal of Clinical Microbiology 49(1): 263-268.
Kiewitz, C., Tummler, B. 2000. Sequence diversity of Pseudomonas aeruginosa: impact on population structure and genome evolution. Journal of Bacteriology 182:3125–3135.
Lang, A.B., Horn, M.P., Imboden, M.A., Zuercher, A.W. 2004. Prophylaxis and therapy of Pseudomonas aeruginosa infection in cystic fibrosis and immunocompromised patients. Vaccine 6 (22) Suppl 1:S44-8.
Maatallah, M., Cheriaa, J., Backhrouf, A., Iversen, A., Grundmann, H., et al. (2011) Population Structure of Pseudomonas aeruginosa from Five Mediterranean Countries: Evidence for Frequent Recombination and Epidemic Occurrence of CC235. PLoS ONE 6(10): e25617. doi:10.1371/journal.pone.0025617.
Micek, S.T., Lloyd, A.E., Ritchie, D.J., Reichley, R.M., Fraser V.J., Kollef, M.H. 2005. Pseudomonas aeruginosa bloodstream infection: Importance of appropriate initial antimicrobial treatment. Antimicrobial Agent and Chemotherapy 49: 1306-1311.
Morales-Espinosa, R., SoberÓn-Chávez, G., Delgado-Sapién, G., Sandner-Miranda, L., Méndez, J.L., González-Valencia, G., Cravioto, A. 2012. Genetic and Phenotypic characterization of a Pseudomonas aeruginosa population with high frequency of genomic islands. PLoS ONE 7(5): e37459.doi:10.371/journal.pone.0037459.
Ndip, R.N., Beeching E.C., Ndip, L.M., Mbacham, W.F., Titanji, V.P.K. (2007). Molecular characterization of Pseudomonas aeruginosa recovered in the Buea Health District of Cameroon: Implications for nosocomial spread. West African Journal of Medicine 26 (3): 191-195.
Oni, A.A., Nwaorgu, O.G.B., Bakare, R.A., Ogunkunle, M.O., Toki, R.A. 2002. The Discharging Ears in Adults in Ibadan, Nigeria; Causative Agents and Antimicrobial Sensitivity Pattern. African Journal of Clinical Experimental Microbiology (3) 3-5.
Osazuwa, F., Osazuwa, E.O, Imade, P.E, Dirisu, J.O, Omoregie, R., et al. 2011. Occurrence of extended spectrum beta-lactamase producing gram negative bacteria in HIV AIDS infected patients with urinary and gastrointestinal tract infections in Benin metropolis. Research Journal of Pharmaceutical, Biological and Chemical Sciences 2: 230-234.
Rolf, F.J. 2000. NTSYS-pc: Numerical Taxonomy and Multivariate Analysis System, version 2.1. Exeter Software, Setauket, New York. http://www.sciencesoftware.
Salama, M.S., Abu Shady H. M., El-Gameal, M. M. B., El Anany, M.G., Abd-El-Rehem, G.M. 2012. Molecular study on relatedness between clinical and tap water isolates of Pseudomonas aeruginosa in two burn units. Life Science Journal 9(1):662-666.
Salimi, H., Owlia, P., Yakhchali, B., Lari, A.R. 2010. Characterization of Pseudomonas aeruginosa in Burn Patients Using PCR- Restriction Fragment Length Polymorphism and Random Amplified Polymorphic DNA analysis. Iran Journal of Medical Sciences 35 (3): 236-241.
Savaþ, L.T.F., Duran, N., Savaþ, N., Nlen, Y., Ocak, S. 2005. The prevalence and resistance patterns of Pseudomonas aeruginosa in intensive care units in a university hospital. Turkish Journal of Medical Sciences 35: 317-322.
Smith, S., Ganiyu, O., John, R., Fowora, M., Akinsinde, K., Odeigah, P. 2012. Antimicrobial Resistance and Molecular Typing of Pseudomonas aeruginosa Isolated from Surgical Wounds in Lagos, Nigeria. Acta Medica Iranica 50 (6).
Stover, C.K., Pham, X.Q., Erwin, A.L., Mizoguchi, S.D. Warrener, P., Hickey, M.J., Brinkman, F.S., Hufnagle, W.O., Kowalik, D.J., Lagrou, M., Garber, R.L., Goltry, L., Tolentino, E., Westbrock-Wadman, S., Yuan, Y., Brody, L.L., Coulter, S.N., Folger, K.R., Kas, A., Larbig, K., Lim, R., Smith, K., Spencer, D., Wong, G.K., Wu, Z., Paulsen, I.T., Reizer, J., Saier, M.H., Hancock, R.E. , Lory, S. and Olson, M.V. 2000. Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen. Nature 406: 959-964.
Taheri, Z.M., Shahbazi, N., Khoddami, M. 2008. Genetic Diversity of Pseudomonas aeruginosa Strains Isolated from Hospitalized Patients. Tanaffos 7(1), 32-39.
Trautmann, M., Halder, S., Lepper, P.M., Exner, M. 2009. Reservoirs of Pseudomonas aeruginosa in the intensive care unit. The role of tap water as a source of infection. Bundesgesundheitsblatt Gesundheitsforschung Gesundheitsschutz 52(3):339-44.
Wolfgang, M.C., Kulasekara, B.R., Liang X., Boyd, D., Wu, K., Yang, Q., Miyada, C.G. 2003. Conservation of genome content and virulence determinants among clinical and environmental isolates of Pseudomonas aeruginosa. Proceedings of National Academy of Science USA 100(14): 8484-8489.
