IDENTIFICATION AND GENETIC DIVERSITY OF TOMATO (Solanum lycopersicum L.) GERMPLASMS IN IBADAN METROPOLIS, NIGERIA
Keywords:
Tomato, landrace, classification, molecular biology, crop improvementAbstract
The adoption of invasive tomato cultivars by peasant farmers has led to a decline in the cultivation of landraces. Presently, a huge number of tomatoes in Ibadan, Oyo State, Nigeria are either hybridized or foreign cultivars which are difficult to identify and classify. It is likely that new varieties may have arisen through anthropogenic influences and natural hybridization. This negligence of landraces has contributed to the limitation on the extent of their genetic diversity for effective breeding and conservation for present and future uses. Consequently, six tomato germplasms were collected from local farmers in Ibadan and had their genetic diversity assessed for improvement, identification, and documentation purposes. The germplasms had their young leaves subjected to DNA extraction, PCR analysis, Sanger sequencing at Inqaba Biotec West Africa, Ibadan. Basic Local Alignment Search Tool (BLAST) was used to confirm the identity of the landraces on the NCBI Genbank. The nucleotide sequences submitted to GenBank were assigned accession numbers: OR809185.1, OR809186.1, OR809187.1, OR809188.1, OR809189.1, and OR809190.1. The Bootstrap phylogenetic tree revealed genetic and evolutionary variation among accessions. The result of BLAST confirmed the identities of the germplasms with accessions in the Genbank as Grape tomato, Heirloom tomato, Red Brandywine, Red Beefsteak, and Cherry tomato for OR809185.1, OR809186.1, OR809187.1, OR809188.1, OR809189.1, and OR809190.1, respectively. The finding showed that variation by phylogenetic tree could be a potential source of genetic diversity for tomato crop improvement. It also documented Grape tomato, Grape tomato, Heirloom tomato, Red Brandywine, Red Beefsteak, and Cherry tomato as tomato varieties available in Ibadan metropolis.
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