HAEMATOLOGICAL STUDIES AND DEOXYRIBONUCLEIC ACID (DNA) CONTENT OF DIPLOID AND TRIPLOID AFRICAN CATFISH Heterobranchus bidorsalis (Geoffroy St. Hilaire 1809)
DOI:
https://doi.org/10.51406/jagse.v10i1.1019Keywords:
Haematology, Triploid, diploid, Deoxyribonucleic acid, Heterobranchus bidorsalisAbstract
This study was carried out to compare some haematological parameters and Deoxyribonucleic acid(DNA) contents of triploid and diploid strains of Heterobranchus bidorsalis. The results showed thatthere were positive correlations among packed cell volume (PCV), Haemoglobin (HB), Red blood cell(RBC) and White blood cell (WBC) at 5% level of significance. Comparatively, diploids had highervalues of WBC, lymphocytes, Mean corpuscular haemoglobin concentration (MCHC) and Mean corpuscularvolume (MCV) but the triploid had higher values with respect to RBC, PCV and HB. Thesevalues showed the effects of the cold shock on the triploid strain. There were no significant differences(p>0.05) between diploid and triploid in the lymphocytes, MCV and MCHC. Variations in otherhaematological values of the diploids and triploids were very minimal. The above observation revealedthat the blood components of diploid and triploid H. bidorsalis were apparently healthy. TheDNA content values obtained in this study could be used as an index to establish ploidy level. Higherpurity of DNA concentration (0.517μg) was found in triploid compared to the diploid concentration of(0.485μg). There was a significant difference (p<0.01) between the triploid and diploid DNA purity.References
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Obafemi Awolowo University, Ile- ife. Pp
154-162.
APHA (Association Public Health Analysts)
1990. Standard methods for the examination
of water and waste water. APHA Washington
DC 14th (ed): 1076p.
Blaxhall, P.C. 1972. The haematological
assessment of the health of freshwater fish.
A review of selected literatures. J. Fish Biol.,
4: 595-604.
Blaxhall, P.C., Daisley, K.W. 1973. Routine
haematology methods for use with fish
blood J. Fish Biol., 5: 771-781.
Ezeri, G.N.O. 2001. Haematological response
of Clarias gariepinus to bacteria infection
and prophylactic treatment with antibiotic.
J. Aquat. Sc., 16: 22-24.
FAO, Food and Agricultural Organisation
1983. Fish feeds and feeding in developing
countries – An International Report on the
ADCP Feed Development programmes,
ADCP/REP/83/18, 97. (1983).
Faturoti, E.O, Balogun, A.M., Ugwu
L.C. 1998. Nutrient utilization and growth
responses of Clarias gariepinus fed different
protein levels. Nig. J. Appl. Fish Hydrol.,
1: 31-49.
Gabriel, U.U. Ezeri, G.N.O., Opabunmi
O.O. 2004. Influence of sex, source, health
status and acclimation on the haematology
of Clarias gariepinus. African Jour. of Biotechnology,
3(9): 463-467.
Kelenyi, G., Neimeith, A. 1969. Comparative
biochemistry and electron microscopy
of the eosinophil leucocyte of vertebrates. A
study of the avian, reptile, amphibian and
fish leucocytes. Acta. Biol. Hung, 20: 405-422.
Macgregor, H.C. 1993. An introduction to
animal cytogenetics. First ed. Chapman & Hall,
2-6 Boundary Row, London SE1 8HN,
U.K pp 72-83.
Omoregie, E., Oyebanji O. 2002. Oxytetracycline-
induced blood disorder in Nile tilapia,
Oreochromis niloticus. J. World Aquaculture
Soc., 33(3): 377-382.
Rambhaskar, B., Rao, K.S. 1990. Use of
haematological parameters as diagnostic
tools in determining the health of milkfish,
Chanos chanos (Forsskal), in brackish culture.
Aqua. and Fisheries Manag., 21: 125-129.
Seiverd, C.E. 1964. Haematology for medical
Technologist. Lea and Fabiger, Philadelphia
P. 946.
Tave, D. 1992. Genetics for Fish Hatchery Managers.
Second edition, Van Nostrand Reinhold,
New York. 415pp.
Weinreb, E.L. 1958. Studies on the histology
and histopathology of the rainbow
trout, Salmo gairdneri Haematology: under
normal and experimental conditions of inflammation
in rainbow trout. Zoologica, N.Y.
46: 145-153.
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